Protein_Domain

Part:BBa_K287001:Design

Designed by: Carlos Andres Sanchez   Group: iGEM09_IPOC1-Colombia   (2009-10-21)


Chloride Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1332
    Illegal SpeI site found at 216
    Illegal PstI site found at 228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1332
    Illegal SpeI site found at 216
    Illegal PstI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1332
    Illegal BglII site found at 3
    Illegal BamHI site found at 1323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1332
    Illegal SpeI site found at 216
    Illegal PstI site found at 228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1332
    Illegal SpeI site found at 216
    Illegal PstI site found at 228
    Illegal AgeI site found at 1200
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 372
    Illegal SapI.rc site found at 420


Design Notes

None


Source

Synthetic gene was cloned into EcoRV digested pBluescript II SK

References